![]() You might get some different colors due to variation in pH, but other than that, you're ok. The ladder will be separated fine, and it will transfer fine - so if all you're using it for is to tell when your gel has run far enough and whether transfer has occurred sufficiently, any pre-stained ladder will be fine. This only comes into play if you're using the standards to find the molecular weight of your protein. TIANGEN Pre-stained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verification of Western Blot. Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. However, as I said, the migration of the pre-stained standards is sensitive to pH, so you'd need to calibrate your pre-stained standard with a non-stained standard to get the apparent molecular weights as they behave in your system. There's no reason why any ladder would not work with this gel system. This gel uses chloride as the mobile anion other gel chemistries use other anions. It usually has a stacking gel of pH 6.8 and a separating gel of pH 8.8. Sizing estimation of proteins on SDS-PAGE and Western blots. A Tris-HCL gel is just the original Laemmli gel, around since the 1970's. Monitoring protein transfer efficiency on membranes after Western blot transferring.
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